NK3R (Neurokinin 3 Receptor) Antibody 20061 from ImmunoStar, Inc.

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Nature communications
October 19 2018

Fig 1: Effects of different selective neurokinin receptor agonists on action potential duration. a APs elicited at 2 Hz from an atrial cardiomyocyte under control conditions (Control) and in the presence of 100 nM NK-1R agonist [Sar9,Met(O2)11]-SP. Bar graph shows average values for AP duration at 20, 50 and 90% of repolarization (APD20, APD50 and APD90) before (Control) and after application of 100 nM [Sar9,Met(O2)11]-SP (N = 6, n = 7, paired t-test). b Atrial APs elicited at 2 Hz in Control and in the presence of the NK-2R agonist (ß-Ala)-NKA(4-10) (100 nM). Bar graph shows average values for APD20, APD50, and APD90 before (Control) and after application of 100 nM (ß -Ala)-NKA(4-10) (N = 3, n = 4, paired t-test). c Atrial APs elicited at 2 Hz in Control and in the presence of the NK-3R agonist Senktide (100 nM). Bar graph shows average values for APD20, APD50 and APD90 before (Control) and after application of 100 nM Senktide (N = 7, n = 16, paired t-test). d Concentration-response curve for increase of APD induced by Senktide in the absence (N = 13, n = 9–16, one-way RM ANOVA) and presence of 300 nM Osanetant (N = 3, n = 3–5, two-way RM ANOVA). Dotted lines: concentration of Senktide at which APD90 prolongation exceeded 20%. All values shown are mean ± SEM. *P < 0.05
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Nature communications
October 19 2018

Fig 2: Expression of the NK-3 receptor and KCNK gene family in left atrium of rabbit and human. a Bar graph showing the normalized tag counts from RNA-seq for the TACR gene and KCNK gene family in the left atrial free wall (LAFW) and the left atrial appendage (LAA) of rabbit (n = 1). Right inset: Scatter plot showing the relation between expression profiles of all genes in LAWF and LAA in rabbit. The blue and red dots indicate TACR and KCNK genes, respectively. b Sections of rabbit (upper panel) and human (lower panel) left atrium showing labelling of the NK-3R (green) in the peripheral sarcolemma of cardiomyocytes, co-stained for the intracellular myocardial marker a-actinin (red). Scale bar 25 µM. Negative controls for immunohistochemistry are shown in Supplementary Figure 4
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Nature communications
October 19 2018

Fig 3: Effect of NK-3R stimulation on ERP and AF inducibility in atria of human and rabbit. a Unipolar electrograms recorded from the left atrium of a Langendorff-perfused rabbit heart before (Control) and after application of 20 nM of Senktide (left). Bar graph showing average values for AERP in Control, in the presence of 20 nM Senktide, and after wash-out (right, N = 7, one-way RM ANOVA). b Unipolar electrograms recorded from human LAA before (Control) and after application of 100 nM of Senktide (left). Bar graph showing average values for AERP in Control and in the presence of 50–200 nmol/L Senktide (right, N = 5, paired t-test, p = 0.06). c Unipolar electrograms recorded from the left atrium of a rabbit Langendorff-perfused heart following a burst pacing protocol in the absence (Control, left) and in the presence of 20 nM of the NK-3R agonist Senktide (right), at baseline (0 cm H2O, upper tracings) and during atrial dilatation (20 cm H2O, lower tracings). d, e Bar graph showing average values for total duration of arrhythmia episodes d and AF incidence e in Control, after application of 20 nM Senktide, and after wash-out (N = 7, two-way RM ANOVA). All values shown are mean ± SEM. *P < 0.05. Electrograms: A atrial activation; V ventricular activation; * stimulus

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NK3R (Neurokinin 3 Receptor) Antibody

ImmunoStar, Inc.'s NK3R (Neurokinin 3 Receptor) Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Immunocytochemistry, Immunofluorescence, and Immunohistochemistry. The NK3R (Neurokinin 3 Receptor) Antibody was generated using TACR3 as the antigen and it reacts with Rat.

Description

The NK3R antiserum was quality control tested using standard immunohistochemical methods in rat hypothalamus using biotin/avidin-HRP techniques.Specificity of the antiserum was demonstrated by soluble pre-adsorption and Western blot. Tissue staining is completely eliminated by pretreatment of the diluted antibody with 25 mg of rat NK3R peptide residues 438-452. Western blot analysis of crude rat brain homogenate demonstrates two immunoreactive bands of approximately 80 and 115 kD

Nature communications

Nature communications

Nature communications

NK3R (Neurokinin 3 Receptor) Antibody from ImmunoStar, Inc.

Description

ImmunoStar, Inc.

Citations (1)

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NK3R (Neurokinin 3 Receptor) Antibody from ImmunoStar, Inc.

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